Fig. 2.

PFE0055c selectively stimulates the ATPase activity of PfHsp70-x. (a) The bar graphs show the basal (grey; set as 1.0) and fold increase in PFE0055c-stimulated (black) ATPase activities of PfHsp70-x and hHsp70 expressed as the mean ± SEM. (b) The bar graphs show the basal (grey; set as 1.0) and fold increase in PFA0660w-stimulated (black) ATPase activities of PfHsp70-x and hHsp70 expressed as the mean ± SEM. In A and B, constituents that were either included or omitted from the reaction medium are indicated by (+) or (−) signs, respectively. Shown here are the combined data from three independent experiments performed in triplicate using independently purified proteins for each experiment. (c) The bar graphs show the stimulation of the basal ATPase activity of PfHsp70-x (0.4 μM; black) and hHsp70 (0.4 μM; grey) with increasing concentrations of PFE0055c (0–2 μM) expressed as the mean ± SEM. Error bars are indicated and an asterisk (*) indicates statistical significance at P < 0.05 relative to the basal ATPase value for the respective chaperone using the Student t test. Shown here are the combined data from at least two independent experiments performed in triplicate using independently purified proteins for each experiment