Figure 4.

IL10-DC + IL35-DC vaccination reduced established CIA clinical scores. CIA was induced with an i.d. injection at the base of the tail of 100 μg of chicken collagen II emulsified in Complete Freund's Adjuvant (5 mg/mL) (CII-CFA). At day 18, when 80% of mice were showing at least mild scores of the disease, mice were vaccinated with 5 × 10⁶ CII-pulsed DCs (CII-DC). Treatment was repeated 2 days later. Arthritis severity was evaluated daily and each paw was individually scored for erythema, swelling, and ankylosis. (A) Schematic timeline of the experimental procedures. (B) Cumulative arthritis scores; CII-DC transfers are indicated by the arrows. (C) Probability of recovery evaluation of mice after the second DC transfer based on cumulative score reduction: each mouse was analyzed for having its scores reduced or not. (D) Individual score evolution of sick mice after the second DC transfer: each line represents the increase (full lines) or decrease (dashed lines) of the cumulative score (y axis) of one individual during days 21 to 28 (x axis). In a second approach, 1 week after immunization, five groups of mice were implanted with bioactive cellular implants secreting IL-10, IL-35, IL-10, and IL-35, or empty implants. (E) Schematic timeline of the experimental procedures. (F) Cumulative arthritis scores. (G) Individual score evolution of sick mice 1 week after the implantation of device: each line represents the increase (full lines) or decrease (dashed lines) of the cumulative score (y axis) of one individual during days 14 to 26 (x axis). Data are representative of at least two independent experiments (n = 3–10 mice/group/experiment). *P < 0.05.