Figure 6.

IL10-DCs in combination with IL35-DCs arrest memory expansion through LAG-3. CD4⁺ T cells were isolated from the spleens of OVA-immunized mice, labeled with a proliferation dye, and put in co-culture with OVA-pulsed DCs in the presence of anti-LAG-3 blocking antibody for 3 day. Flow cytometric analysis of (A) T cell proliferation after co-cultures and (B) CD4⁺ memory (CD62L⁺CD44^high) or effector (CD62L⁻CD44^high) cell population. Data are representative of at least two independent experiments with T cells isolated from five independently immunized mice (n = 5, each experiment). **P < 0.005, ***P < 0.001, ****P < 0.0001.