FIGURE 4.

Effect of cmp4 on cell viability and Ras signaling of human breast cancer MDA-MB-231 cell line expressing KRas^G13D. (A) Growth curves of MDA-MB-231 treated with increasing concentration of cmp4 or vehicle (deionized water) supplemented in the growth medium. After 24, 48, and 72 h of treatment cells were trypsinized and counted with a Burker chamber. (B) Microscopy analysis of MDA-MB-231 treated for 48 h with different concentrations of cmp4. (C) Cell viability of MDA-MB-231 cells treated with increasing concentration of cmp4, or vehicle (deionized water) for 24, 48, and 72 h as measured by MTT assay; data were normalized on cells treated with vehicle imposed as equal to 1. (D) Phosphorylated MAPK level in cell lysates from MDA-MB-231 cells no treated or 24 h-treated with cmp4 or vehicle. Data were normalized on the phospho-MAPK level in MDA-MB-231 treated with vehicle imposed as equal to 1. Data shown are mean and standard deviation of two independent experiments, each performed in triplicate. Single and double asterisk above histograms indicates a statistical significance of 95% and 99% respectively, calculated by Student’s t-test in comparison to cells treated with vehicle.