FIGURE 7.

Effect of 72 h-treatment with cmp4 and/or Cetuximab (CTX) on cell viability of human colorectal cancer SW48 isogenic cell lines. (A–C) Relative cell viability of SW48 KRAS ^WT/WT, SW48 KRAS ^WT/G12V , and SW48 KRAS ^WT/G13D cells treated for 72 h with different concentrations of CTX (A) or cmp4 (B). (C) Results from the simulations of the SW48 KRAS ^WT/WT, SW48 KRAS ^WT/G12V and SW48 KRAS ^WT/G13D mathematical models either untreated or treated with the following drugs: 0.5 nM CTX (corresponding to an inhibition of nearly 70% of GEF activity); 100 µM cmp4, which is around IC₅₀ for this compound on multilevel mechanisms of action; a combination of both. (D) Relative cell viability of SW48 KRAS ^WT/WT, SW48 KRAS ^WT/G12V, and SW48 KRAS ^WT/G13D cells treated for 72 h either with 0.5 nM CTX, 100 μM cmp4, or a combination of both. Data were normalized on cells treated with vehicle taken equal to 1. Single, double, and triple asterisk above histograms in (A,B,D) indicates a statistical significance of 95%, 99%, and 99.9% respectively, calculated by Student’s t-test in comparison to cells treated with vehicle.