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. 2021 Feb 8;22(3):e50629. doi: 10.15252/embr.202050629

Figure EV1. FUNDC1 is regulated by PGC‐1α.

Figure EV1

  • A–C
    Oil red O staining (A) analysis of differentiated brown adipocytes (5 days) and Western blotting analysis (B) of brown preadipocytes (0 days) and differentiated cells (5 days), scale bar, 10 μm. The expression levels of the indicated proteins are quantified in (C). Data information: experiments were repeated three times, data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. ns: no significant difference; *P < 0.05.
  • D–F
    Brown fat preadipocytes were infected with control adenovirus and adenovirus expressing PGC‐1α. GFP expression was used as an indicator for cell infection, scale bar, 10 μm (D). Levels of the indicated proteins were analyzed by Western blotting and quantified (E, F). Data information: experiments were repeated three times, data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. ns: no significant difference; *P < 0.05.
  • G, H
    Western blotting analysis of the indicated proteins in HeLa (G) and 293 (H) cells transfected with control vector and a Flag‐PGC‐1α expression construct. Quantification of the expression levels of the indicated proteins is shown in the panel below. Data information: Experiments were repeated three times, and data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. *P < 0.05.