-
A–C
Western blotting analysis of the indicated proteins in scramble, PGC‐1α knockdown, and PGC‐1β knockdown brown fat preadipocytes (A). Expression levels of the indicated proteins were quantified (B). Real‐time PCR analysis of the expression of the indicated genes in scramble, PGC‐1α knockdown, and PGC‐1β knockdown brown fat preadipocytes (C). Data information: Experiments were repeated three times, and data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. ns: no significant difference; *P < 0.05; **P < 0.01; ***P < 0.001.
-
D, E
Scramble and NRF1 knockdown preadipocytes were induced to differentiate into mature adipocytes, and samples were collected for Western blotting analyses and quantification of the indicated proteins. Data information: Experiments were repeated three times, and data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. ns: no significant difference; **P < 0.01.
-
F
Expression of the indicated genes was analyzed by real‐time PCR in BAT before and after cold exposure (4°C) for 72 h. Data information: n = 3 biological replicates, data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. *P < 0.05.
-
G, H
The WT mice were treated with chloroquine (CQ; 30 mg/kg/day) for 72h in normal or cold conditions, and the expression of LC3B was detected by Western blotting. The expression of LC3BII was quantified and plotted (H). Data information: n = 6 biological replicates, data are represented as the mean ± SEM. Statistical analysis was performed using two‐way ANOVA test. **P < 0.01.
