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. 2021 Feb 8;22(3):e50629. doi: 10.15252/embr.202050629

Figure EV5. FUNDC1‐dependent mitophagy controls mitochondrial quality.

Figure EV5

  1. Mitochondrial ROS levels were measured by Mito‐Sox staining in WT and Fundc1 knockout (F1 KO) brown fat preadipocytes. Data information: Experiments were repeated four times, and data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. **P < 0.01.
  2. ATP levels were measured with a luciferase assay kit in WT and Fundc1 KO preadipocytes. Data information: Experiments were repeated three times, and data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. **P < 0.01.
  3. OCR was measured in WT and Fundc1 KO preadipocytes. Data information: Experiments were repeated four times, and data are represented as the mean ± SEM. Statistical analysis was performed using two‐tailed Student's t‐test. *P < 0.05; **P < 0.01; ***P < 0.001.
  4. Oil red O staining analysis of wild‐type and Fundc1 KO brown fat cells after differentiation for 5 days, scale bar, 10 μm.