Figure EV1. The lack of mavQ and lepB does not affect the intracellular replication of L. pneumophila .

BMDMs infected with indicated L. pneumophila strains were lysed with saponin at 2, 24, 48, and 72 h post‐infection. The saponin‐solubilized lysates of infected cells were plated on CYE plates and grown at 37°C for 4 days to determine the CFUs. Results are shown as mean ± SD (technical replicates, n = 3) and are representative of three independent experiments (biological replicates, n = 3).