Figure EV4. LegA5 is not required for the anchoring of SidC to the LCV.

1. Representative immunofluorescence images of anti‐Legionella and anti‐SidC staining of bacterial phagosomes. BMDMs were infected with the indicated L. pneumophila stains at an MOI of 2 for 2 h. SidC association on the bacterial phagosomes was detected as described in Fig 5. Insets represent 3× magnification of regions defined by dash lines. Scale bar, 5 μm.
2. Percentages of SidC‐positive LCVs. At least 100 phagosomes (n ≥ 100) were scored for each sample. Results are shown as mean ± SD of three independent experiments (biological replicates, n = 3). Unpaired two‐tailed Student’s t‐test, p < 0.05 indicates significant difference.