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. 2021 Feb 15;22(3):e52164. doi: 10.15252/embr.202052164

Figure 4. Upon inhibition of CUL2LRR1, CMG persists on chromatin throughout interphase and is removed during early mitosis.

Figure 4

  • A
    Time‐lapse analysis of mouse ES cells expressing GFP‐SLD5 and mCherry‐PCNA, following inhibition of CUL2LRR1. Arrows illustrate the persistence of GFP‐SLD5 on heterochromatin after disappearance of mCherry‐PCNA.
  • B
    Similar time‐lapse analysis of mouse ES cells expressing GFP‐SLD5 and mCherry‐Histone H2B (from the CAG promoter at the ROSA26 locus—see Materials and Methods), after inhibition of CUL2LRR1. Arrows indicate heterochromatic patches of GFP‐SLD5 that disappear during entry into mitosis (98% cells, n = 97).
  • C
    Equivalent data to (B) but for untreated cells (100% of untreated cells lacked GFP‐SLD5 on mitotic chromatin, n = 149).

Data information: Scale bars in all panels correspond to 10 µm.