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. 2021 Feb 15;22(3):e52164. doi: 10.15252/embr.202052164

Figure EV5. Models for disassembly of the metazoan replisome. The figure summarises data from this manuscript together with previous studies with early embryos of Caenorhabditis elegans and extracts of Xenopus laevis eggs.

Figure EV5

  • A
    During DNA replication termination, CUL2LRR1 promotes ubiquitylation of CMG‐MCM7, leading to disassembly by p97‐UFD1‐NPL4.
  • B
    In cells that lack CUL2LRR1 activity, the CMG replisome remains on chromatin after DNA replication termination. Upon entry into mitosis, the TRAIP ubiquitin ligase activates a second pathway of CMG disassembly, dependent upon p97.
  • C
    Previous work showed that TRAIP is required in human cells to process sites of incomplete DNA replication during mitosis, by a process of Mitotic DNA Synthesis or “MiDAS” (Sonneville et al, 2019). It is likely that the first step in this pathway is TRAIP‐dependent CMG disassembly upon entry into mitosis.