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. Author manuscript; available in PMC: 2021 Oct 28.
Published in final edited form as: Sci Transl Med. 2020 Oct 28;12(567):eabb7656. doi: 10.1126/scitranslmed.abb7656

Fig. 5. Weekly doses of 500 mg/kg of BNZ is sufficient to eliminate T. cruzi in heart and skeletal muscle.

Fig. 5.

C57BL/6 wild-type mice were intraperitoneally infected with 4×106 tdTomato-expressing Colombiana trypomastigotes of T. cruzi and treated weekly for 7 weeks, with 100 or 500 mg/kg of BNZ, starting at 5 days post-infection. At each indicated time point, one mouse per group was sacrificed, perfused and heart and skeletal muscle were dissected, clarified and scanned using LSFM. (A) 3D reconstructions of heart and skeletal muscle showing amastigote nests (red) in untreated (0 weeks) and treated (8 weeks) mice. Arrows indicate amastigote nests. (B) Automated quantification of total T. cruzi amastigote nests in tissue 3D reconstructions. ND = not determined.