Table 3.
Quantification (mg/g of extract) of the phenolic and non-phenolic compounds present in tisanes prepared from five MAP mixtures (mean ± SD).
| Peak | T1 | T2 | T3 | T4 | T5 |
t-Students Test p-Value |
|---|---|---|---|---|---|---|
| 1 A | 0.41 ± 0.01 | nd | nd | nd | nd | - |
| 2 B | nd | nd | nd | nd | 3.13 ± 0.14 | - |
| 3 C | nd | 0.163 ± 0.002 | nd | nd | nd | - |
| 4 D | 0.143 ± 0.001 | nd | nd | 0.151 ± 0.002 | nd | 0.001 |
| 5 E | nd | 0.863 ± 0.01 | nd | 0.29 ± 0.01 | nd | <0.001 |
| 6 B | nd | nd | 0.69 ± 0.02 | nd | 0.63 ± 0.03 | <0.001 |
| 7 F | 0.172 ± 0.002 | nd | nd | nd | nd | - |
| 8 G | nd | 0.086 ± 0.004 | nd | 0.115 ± 0.004 | nd | - |
| 9 B | nd | nd | 1.19 ± 0.02 | nd | 30.88 ± 1.17 | <0.001 |
| 10 B | nd | nd | nd | nd | 3.66 ± 0.01 | - |
| 11 D | nd | 0.215 ± 0.004 | nd | nd | nd | - |
| 12 B | nd | nd | nd | nd | 2.07 ± 0.06 | - |
| 13 H | nd | 1.016 ± 0.003 a | nd | 0.606 ± 0.002 b | 0.442 ± 0.001 c | - |
| 14 A | 0.1303 ± 0.002 | nd | 0.15 ± 0.01 | nd | nd | 0.001 |
| 15 I | nd | nd | nd | nd | 1.65 ± 0.03 | - |
| 16 E | nd | 1.07 ± 0.03 | nd | nd | nd | - |
| 17 I | nd | nd | nd | nd | 1.98 ± 0.76 | - |
| 18 A | 2.69 ± 0.05 | nd | nd | 0.263 ± 0.004 | nd | <0.001 |
| 19 A | 0.82 ± 0.01 | nd | nd | 0.49 ± 0.02 | nd | <0.001 |
| 20 J | nd | tr | nd | nd | nd | - |
| 21 J | nd | nd | 2.47 ± 0.04 | 2.27 ± 0.08 | nd | <0.001 |
| 22 K | 2.33 ± 0.01 | nd | nd | 1.29 ± 0.01 | nd | <0.001 |
| 23 K | nd | nd | 0.747 ± 0.004 | nd | nd | - |
| 24 G | nd | nd | 6.49 ± 0.23 | 5.77 ± 0.12 | nd | <0.001 |
| 25 J | nd | 1.52 ± 0.03 | nd | nd | 0.90 ± 0.01 | <0.001 |
| 26 L | nd | 4.58 ± 0.18 | nd | nd | nd | - |
| 27 G | nd | nd | nd | 1.996 ± 0.003 | 3.17 ± 0.07 | - |
| 28 J | nd | nd | nd | 1.19 ± 0.01 | nd | - |
| 29 K | nd | 1.99 ± 0.01 | nd | nd | nd | - |
| 30 J | 0.23 ± 0.003 | nd | nd | 1.59 ± 0.01 | nd | - |
| 31 J | nd | nd | nd | 3.533 ± 0.003 | nd | - |
| 32 J | nd | nd | 5.91 ± 0.02 | nd | nd | - |
| 33 J | nd | nd | nd | nd | 2.29 ± 0.01 | - |
| 34 L | nd | 8.73 ± 0.11 d | 7.05 ± 0.13 c | 10.08 ± 0.22 b | 15.92 ± 0.34 a | - |
| 35 L | nd | 3.17 ± 0.04 | nd | nd | 8.09 ± 0.25 | <0.001 |
| 36 K | 0.61 ± 0.01 | nd | nd | nd | nd | - |
| 37 F | 0.107 ± 0.004 | nd | nd | nd | nd | - |
| 38 K | nd | 6.12 ± 0.12 | 1.23 ± 0.02 | nd | nd | <0.001 |
| 39 K | 0.828 ± 0.013 c | nd | nd | 1.84 ± 0.05 a | 1.55 ± 0.07 b | - |
| 40 K | nd | nd | 1.71 ± 0.07 | 1.79 ± 0.01 | nd | <0.001 |
| 41 J | 1.19 ± 0.05 | nd | nd | nd | nd | - |
| 42 K | 10.84 ± 0.14 d | 20.78 ± 0.34 b | 22.65 ± 0.33 a | 20.66 ± 0.26 b | 19.9 ± 0.13 c | - |
| 43 K | nd | 9.60 ± 0.39 | nd | nd | nd | - |
| 44 J | nd | nd | 0.278 ± 0.005 | nd | nd | - |
| 45 D | nd | nd | 3.16 ± 0.02 | nd | nd | - |
| 46 J | nd | nd | 1.19 ± 0.04 | 0.88 ± 0.03 | nd | <0.001 |
| 47 J | nd | 0.68 ± 0.01 | nd | nd | nd | - |
| 48 K | nd | nd | 3.34 ± 0.02 | 5.46 ± 0.19 | nd | - |
| 49 K | 6.84 ± 0.09 a | nd | nd | 1.14 ± 0.03 c | 5.61 ± 0.09 b | - |
| 50 M | nd | nd | 1.03 ± 0.01 | nd | nd | - |
| 51 J | nd | nd | nd | 0.40 ± 0.02 | nd | - |
| 52 G | nd | nd | 36.04 ± 0.05 | nd | nd | - |
| 53 K | nd | nd | nd | 0.95 ± 0.01 | nd | - |
| 54 K | 2.59 ± 0.03 | nd | nd | nd | 0.87 ± 0.01 | <0.001 |
| 55 J | nd | nd | nd | 0.080 ± 0.001 | nd | - |
| 56 | nd | nd | nq | nd | nd | - |
| 57 | nd | nd | nd | nd | nq | - |
| 58 | nd | nd | nq | nd | nd | - |
| 59 G | nd | nd | 0.026 ± 0.001 | nd | nd | - |
| 60 K | nd | nd | 1.07 ± 0.06 | nd | nd | - |
| 61 K | nd | nd | 0.25 ± 0.01 | nd | nd | - |
| 62 K | nd | nd | 7.28 ± 0.13 | nd | nd | - |
| 63 K | nd | nd | 9.22 ± 0.21 | nd | nd | - |
| TPA | 28.51 ± 0.28 e | 40.59 ± 0.13 b | 31.73 ± 0.23 d | 34.34 ± 0.10 c | 68.30 ± 0.78 a | - |
| TiF | nd | nd | 17.81 ± 0.14 | nd | nd | - |
| TFa | nd | 0.086 ± 0.004 d | 6.495 ± 0.23 b | 7.88 ± 0.12 a | 3.17 ± 0.07 c | - |
| TFo | 1.42 ± 0.05 e | 19.7 ± 0.3 d | 53.98 ± 0.08 a | 20.58 ± 0.19 c | 27.66 ± 0.08 b | - |
| TF3O | nd | nd | nd | nd | 3.64 ± 0.04 | - |
| TOC | nd | 0.215 ± 0.004 | 3.16 ± 0.02 | nd | nd | <0.001 |
| TPC | 29.93 ± 0.34 e | 60.58 ± 0.45 d | 113.19 ± 0.68 a | 62.79 ± 0.41 c | 102.78 ± 0.84 b | - |
nd—not detected; tr—traces; nq—non quantifiable. TPA—Total Phenolic acids; TiF—Total isoflavones; TFa—Total flavanones: TFo—Total flavonoids; TF3O—Total flavan-3-ol; TOC—Total other compounds; TPC—Total phenolic compounds. Standard calibration curves used for quantification in each peak: A—caffeic acid; B—chlorogenic acid; C—syringic acid; D—cinnamic; E—p-coumaric acid; F—ferulic acid; G—naringenin; H—apigenin-6-C-glucoside; I—catequin; J—quercetin-3-O-rutinoside; K—rosmarinic acid; L—quercetin-3-O-glucoside; and M—apigenin-7-O-glucoside. ANOVA analysis—In each row different letters mean significant differences (p < 0.05).