Figure 5.

Molecules associated with the canonical pathway (CP) ERK/MAPK signaling in the function of two stages of oocyte development. The Ingenuity Knowledge Base defines both CPs. (a) The relationship between MAPKs, RAS, and Hsp27 with oocyte maturation in ERK/MAPK signaling. (b) The relationship between MAPKs with the expansion of the cumulus–oocyte complex in ERK/MAPK signaling. The proteins highlighted with shadowed blocks are identified in three biological samples of canine in vitro oviductal cell-derived extracellular vesicles. The solid gray lines represent the genes associated with a CP. The arrows represent the directionality of the interaction between molecules or a molecule and a bio-function. The solid line is for direct interaction, and the dashed line is for indirect interaction.