Table A2.
Detailed sequence of used primers.
| Name | Sequence | Attached Sequence |
|---|---|---|
| oIB-288F | gatagatctACCATGGGCAAAGCCGGCGGCTGCCG | BglII, Kozak |
| oIB-290R | gaagcggccgcTCATAGTTCATCCTTAGCATAATCTGGAACATCATATGG | NotI, KDEL, HA tag |
| oIB-306R | GTTTGTGGTGGAAAGCCAAAGCGGTAACAGATG | |
| oIB-307F | CATCTGTTACCGCTTTGGCTTTCCACCACAAACtatccatatgatgttccagattatg ctaaggatgaactatg | HA tag, KDEL, reverse complement of oIB-306F |
| oIB-308R (pLPCX primer) |
GGTCCTTGGGGCACCCTGGAAACATC | |
| oIB-311R | AGAAAGGAGCACCTCCACCTGCTTGTG | |
| oIB-312F | gcaggtggaggtgctcctttctgacACAAAGGCCCAGCCTATAGACTCCATATTAG | Reverse complement of oIB-311R |
| oIB-319F (pLPCX primer) |
GGACTTTCCTACTTGGCAGTACATCTACG | |
| oIB-361R | CCTACTCATGGGCGCGGCCC | |
| oIB-362F | gggccgcgcccatgagtaggCGAAGCATCTCAAAGAAGAACACAACCTC | Reverse complement of oIB-361R |
| oIB-363 | TGGAGGCAGCTGCTGCTCCTTC | |
| oIB-364 | gaaggagcagcagctgcctccaGAAGACCAGCCATCTTCTGAAAATGATTCTC | Reverse complement of oIB-363R |
| oIB-315F | gatctcgagACCATGGCTGCCTGCGAGGGCAG | XhoI, Kozak |
| oIB-316R | gaagcggccgcTTAAGCATAATCTGGAACATCATATGGATAGGCAGGGG TCCAGATAGGCAGAGG | NotI, HA tag |