Skip to main content
. 2021 Feb 23;22(4):2226. doi: 10.3390/ijms22042226

Figure 7.

Figure 7

Insertion of GGMP repeat residues into DnaK did not result in PfHop binding. Slot blot and ELISA were conducted to explore interaction of DnaK-G with PfHop. The slot blot images and graphs generated from ELISA absorbance readings to explore interaction of PfHop with DnaK and its variant, DnaK-G in the absence of nucleotides (A), and in the presence of either 5 mM ATP (B), or 5 mM ADP (C), respectively. The relative intensities for PfHop-DnaK-G interaction were normalized relative to intensity of signal obtained for DnaK-PfHop at their highest amounts of the proteins for the assay conducted in the absence of nucleotides (D). The error bars represent data from three assays. Two-way ANOVA was used to determine the statistical significance at (* p < 0.05; ** p < 0.01; *** p < 0.001).