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. 2021 Jan 20;10(3):386. doi: 10.3390/jcm10030386

Figure 2.

Figure 2

Effects of GLY extracts on the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation. (A) Microscopic observations for tartrate-resistant acid phosphatase (TRAP) staining of osteoclast-like positive multinucleated cells (MNCs) derived from soluble RANKL (sRANKL)-treated RAW264.7 cells in the absence or presence of various concentrations of the GLY extracts for five days. (a) no-treated with sRANKL and absences of GLY extracts; (b) absence of GLY extracts; (c) 0.01 mg/mL of GLY extracts; (d) 0.05 mg/mL of GLY extracts; (e) 0.1 mg/mL of GLY extracts; (f) 0.2 mg/mL of GLY extracts. Magnification x100, the arrowheads indicate TRAP-positive osteoclasts. Scale bars represent 300 µm. (B) Quantitative analysis of TRAP-positive MNCs with three or more nuclei in each well. Data are presented as the mean ± SD. ** p < 0.01 compared with the sRANKL treatment in the absence of GLY extracts. These procedures were repeated three times by using triplicate RAW264.7 cell cultures.