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. 2021 Jan 20;10(3):386. doi: 10.3390/jcm10030386

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of GLY extracts on the expression of osteoclast-specific markers in RAW264.7 cells. (A) sRANKL-treated RAW264.7 cells were incubated in the absence or presence of various concentrations (0.05, 0.1, 0.2 and 0.5 mg/mL) of GLY extracts for five days. The expression of NFATc1, cathepsin K and ephrin B2 in the cells were evaluated using Western blot analysis. Β-actin served as a loading control. (B) The expression of DC-STAMP and β-actin in the cells were evaluated using Western blot analysis.