Skip to main content
. 2021 Jan 20;10(3):386. doi: 10.3390/jcm10030386

Figure 5.

Figure 5

The effect of GLY extracts on osteoclast differentiation in ligature-induced periodontitis. Microscopic observations were performed on the decalcified paraffin sections with TRAP staining on day 4 from the placement of ligature. (A) Representative images have shown TRAP-positive osteoclasts in the alveolar bone around maxillary secondary molar in the non-ligatured and ligatured buccal side; upper panels: x100 magnification, lower panels: x200 magnification. Ab, alveolar bone; De, dentin; Pu, pulp; the asterisk indicates the nylon ligature, and the arrowheads indicate TRAP-positive osteoclasts, Scale bars represent 100 µm. (B) Quantitative analysis of TRAP-positive osteoclasts on day 4 in the ligatured buccal and palatal side. TRAP-positive cells per visual field, counted 16–24 visual fields. Data are presented as the mean ± SD from four or six rats per group. Double asterisk indicates p < 0.01. Control; group of oral administration of distilled water for four days (n = 4), GLY admin.; group of oral administration with 60 mg/kg of GLY extracts for four days (n = 6).