Figure 4.

Changes in bacterial growth (colony-forming units (CFU)/mL) in human milk samples inoculated with bacteria strains, freeze-dried, and stored for up to 6 months. The data are presented as mean values with standard deviations. One-way analysis of variance (ANOVA) with post hoc Tukey’s test was used to statistically analyze the data from all replicates; Paired t-tests were used for the initial bacterial concentration (IC) and bacterial concentrations after freeze-drying at time 0 (Lyo 0), at 3 months of storage (Lyo 3), and at 6 months (Lyo 6) of storage. Statistically significant differences are marked with asterisks, depending on the degree of significance: *** p < 0.0001, ** p < 0.001, * p < 0.05, whereas non-significant differences (p > 0.05) are marked with ‘ns’.