Table 1.
Membrane scaffold proteins. A summary of the most used MSPs in nanodisc formation including final nanodisc diameter, molecular mass, and particular features associated with each MSP is shown.
| Membrane Scaffold Protein (MSP) | Nanodisc Diameter (nm) |
Features | Molecular Mass (Da) |
|---|---|---|---|
| MSP1 | 9.7 | Original MSP1 (deletion 1–43 mutant of human Apo A-1) | 24,608 |
| MSP1TEV | 9.7 | MSP1 with removable 7-his tag | 26,930 |
| MSP1D1 | 9.7 | Deletion 1–11 mutant of MSP1TEV | 24,662 |
| MSP1D1 D73C | 9.6 | D73C MSP1D1 mutant in helix 2, Apo A-1 numbering | 24,650 |
| MSP1D1 (-) | 9.6 | MSP1D1 lacking 7-His tag | 22,044 |
| MSP1D2 | 9.6 | MSP1 variant lacking the first helix. | 24,608 |
| MSP1E1 | 10.4–10.6 | Extended MSP1, helix 4 repeated | 27,494 |
| MSP1E2 | 11.1–11.9 | Extended MSP1, helices 4 and 5 repeated | 30,049 |
| MSP1E3 | 12.1–12.9 | Extended MSP1, helices 4, 5, and 6 repeated | 32,546 |
| MSP1E1D1 | 10.5 | Extended MSP1D1, helix 4 repeated | 27,547 |
| MSP1E2D1 | 11.1 | Extended MSP1D1, helices 4 and 5 repeated | 30,103 |
| MSP1E3D1 | 12.1 | Extended MSP1D1, helices 4, 5, and 6 repeated | 32,600 |
| MSP2 | 9.5 | Fusion of two MSP1 with GT-linker | 48,020 |
| MSP2N2 | 15.0–16.5 | Fusion of MSP1D1 and MSP1D2 with GT linker | 45,541 |
| MSP2N3 | 15.2–17 | Fusion of MSP1D1 and MSP1D1-17 (deletion amino acids 1–17) with GT linker | 46,125 |
| MSP1FC | 9.7 | MSP1D1 with C-terminal FLAG-tag | 25,714 |
| MSP1FN | 9.6 | MSP1D1 with N-terminal FLAG-tag | 25,714 |