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. 2021 Feb 23;13(2):352. doi: 10.3390/v13020352

Figure 7.

Figure 7

RSV G protein CX3C-CX3CR1 binding is inhibited by serum IgG from G protein immunogen vaccinated mice. Purified serum IgG from G protein immunogen or adjuvant vaccinated mice was examined for the ability to inhibit (A) FKN or (B) G protein CX3C binding to CX3CR1. IgG was co-incubated with FKN or G protein and 5 μg/mL heparin to block non-specific binding. Ligand-specific binding and percent inhibition was determined by: [1 − (% Alexa Fluor-488+ CX3CR1.293+ cells treated with ligand + antibody mixture) / (% Alexa Fluor-488+ CX3CR1.293 cells treated with ligand + normal mouse IgG)] × 100, as previously described. Bars represent mean of at least three independent experiments + SEM. Analysis by one-way ANOVA compared to adjuvant-only control where, ** p < 0.01, *** p < 0.001 **** p < 0.00001.