Figure 2.

Confocal microscope analysis of macrophage density in the LV of control (a–c) and db/db (d–f) mice. Macrophages were visualized with anti-CD68 antibody. WGA (wheat germ agglutinin) was used to mark microvessels and cardiac myocyte boundaries. Figures were chosen among at least three independent stainings; the number of CD68 positive macrophages per mm² in whole (g) and in specific areas of heart (h) was calculated. Data are expressed as mean number of cells per area (mm²), calculated within three independent stainings and 10 randomly selected regions of interest per staining; RV—right ventricle, LV—left ventricle, S—interventricular septum. * p < 0.05, ** p < 0.01.