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. 2021 Feb 20;26(4):1127. doi: 10.3390/molecules26041127

Figure 8.

Figure 8

Inhibitory effects of BPNs 1-5, PBN, and NAC on Reactive Oxygen Species (ROS) (superoxide) production in SHSY5Y human neuroblastoma cell cultures exposed to OGD (4 h) and 3 h reperfusion (I/R). Bars show the percentage of ROS formed after OGD and IR with, or without, BPNs 1-5 or PBN and NAC, at the indicated concentrations. Values are mean ± SEM of at least three experiments, each one performed in triplicate (n = 3). Values for ROS in basal conditions were calculated as 0.46 ± 0.08 UAF/min/100.000 cells (n = 4). The statistics compares the effect of I/R against controls or the effect of the different compounds respect to I/R at * p < 0.05, ** p < 0.01, *** p < 0.001 (one-way ANOVA followed by Holm–Sidak analysis post hoc).