Figure 9.

Antioxidant effect of BPNs 1–5, PBN, and NAC after I/R in human neuroblastoma SH-SY5Y cells. (A) Dose–response curves showing the percentage of antioxidant effect of different compounds at the indicated concentrations. The curve adjustments to estimate the EC₅₀ were carried out by non-linear ponderated regression analysis of minimal squared, using logistic curves f1 = min+(max-min) /(1+(x/EC₅₀)^(-Hillslope). Data represent mean ± SEM of three experiments, each one done in triplicate (n = 3). The analysis was implemented using the software SigmaPlot v.11. (B) EC₅₀ values and maximal antioxidant activities for the indicated compounds. The statistics compares the differences between EC₅₀ or maximal activities values for different compounds tested against PBN or BPMN2 at * p < 0.05, ** p < 0.01 y *** p < 0.001 (one-way ANOVA, followed by Holm–Sidak analysis as a post hoc test).