Figure 6.

Increasing fibrinogen concentration in cryoprecipitate, but not fibrinogen concentrate, increases clot stability. Clots were formed from 30% FDP and 300 pM tissue plasminogen activator (tPA) spiked with 0.5, 1, 1.5 or 3 mg/mL fibrinogen concentrate (Fg-C; (A)) or cryoprecipitate (cryo; (B)). Clot formation and lysis were monitored by measuring absorbance at 405 nM every 60 s for 4 h and 50 % lysis times were calculated using the Shiny App software for clot lysis [25]. Dotted lines on the y axis represent the 50% lysis time for control samples (PNP). Data are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. n = 6.