Skip to main content
. 2021 Feb 21;13(2):336. doi: 10.3390/v13020336

Figure 2.

Figure 2

Pf4 curing verification. (A-C) Curing verification by PCR, Lanes 1–2 represent the DNA of randomly picked Crb-sensitive colonies, and lane 3 represents the WT PAO1 for control. (A) Amplification of 1800 bp around the Pf4 attB site. (B) Amplification of 1000 bp around the Pf4 attR site that can only be amplified in the integrated form. (C) Amplification of 750 bp around the Pf4 attP site that can only be amplified in the RF form. (D) Curing verification by plaque assay, serial dilutions of Pf4 phage are extracted from WT PAO1 strain used to infect WT PAO1 and ΔPf4 strain.