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. 2021 Feb 23;10(2):478. doi: 10.3390/cells10020478

Figure 1.

Figure 1

IQGAP specifically interacts with the core proteins of the MST/Hippo pathway. HEK 293 cells were grown in 10% serum or serum deprived (0.1% serum) for 16 h. (A) Endogenous LATS1 immunoprecipitates were analysed by western blot with the indicated antibodies. (B) Endogenous MST2 immunoprecipitates were analysed by western blot with the indicated antibodies. (C) Endogenous YAP1 immunoprecipitates were analysed by western blot with the indicated antibodies. IgG indicates isotypic antibody that was used as negative control for unspecific binding to protein G agarose beads. (D) HeLa cells grown in 10% serum or 0.1% serum and endogenous LATS1 or MST2 were immunoprecipitated. IQGAP co-immunoprecipitation was monitored using a specific antibody. Expression of the indicated proteins in cell extracts was detected by blotting with the indicated antibodies. IP blots were quantified using ImageJ and the numbers show relative fold change of IQGAP1 normalised by LATS1, MST2 or YAP1 IP blots, as indicated.