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. 2021 Feb 23;10(2):478. doi: 10.3390/cells10020478

Figure 2.

Figure 2

IQGAP1 scaffolds MST2-LATS1 interaction and regulates the activation of the kinases of the pathway. (A) HEK 293 cells were transfected with the indicated amounts of Myc-IQGAP1 constructs. Endogenous MST2 immunoprecipitates were analysed by western blot with the indicated antibodies. IP blots were quantified using ImageJ and the numbers show relative fold change of LATS1 normalised by MST2. (B) HEK 293 cells were transfected with IQGAP1 siRNA or a non-targeting (Scr.) siRNA pool. Endogenous MST2 and LATS1 immunoprecipitates were examined by western blotting. IQGAP1 and Tubulin blots were quantified using ImageJ and the numbers shows relative fold change of IQGAP1 normalised by Tubulin. IP blots were quantified using ImageJ and the numbers show relative fold change of MST2 normalised by LATS1. (C) HeLa cells were transfected with IQGAP1 siRNA or a non-targeting (Scr.) siRNA pool. Endogenous MST2 immunoprecipitates were examined by western blotting. Blots were quantified as in (A).