Skip to main content
. 2021 Feb 23;10(2):478. doi: 10.3390/cells10020478

Figure 6.

Figure 6

IQGAP1 YAP interactions and YAP-dependent transcription. (A) YAP1 immunoprecipitates from HEK 293 cells co-transfected with the indicated amounts of Myc-IQGAP1 and HA-p73 (1 µg) constructs. HA-p73 co-immunoprecipitation levels were measured by western blot. IP blots were quantified using ImageJ and the numbers show relative fold change of MST2 or LATS1 normalised by IQGAP1 blots. (B) YAP1 immunoprecipitates from HEK 293 cells co-transfected with the indicated amounts of Myc-IQGAP1 construct. TEAD co-immunoprecipitation levels were measured by western blot. (C) Total protein extracts from HEK 293 cells transfected with Flag-YAP1, Flag-YAP1-S127A or the corresponding empty vector analysed by western blot. (D) Upper panel: Luciferase assay of PUMA promoter activity in HEK 293 cells co-transfected with PUMA Frag1-Luc and β-Gal plasmids, and LATS1 kinase dead mutant (KD) or the corresponding empty vector and IQGAP1 siRNA or the corresponding non-targeting siRNA pool. Luciferase activity normalised against β-galactosidase signal. Lower panel: Total lysates corresponding to the luciferase assay measured by western blot. p-values were obtained by Student’s t-test, n = 3, error bars indicate SEM, * = p < 0.05.