Table 1.
Multiple sequence analysis of TMEM163 and ZnT protein family using Clustal Omega (MegAlign Pro, Lasergene v17.1). Percent identity and similarity are shown by the upper right values (bold text) and lower left values (regular text), respectively. The identity percentage signifies the number of amino acids that exactly matches sequences between two proteins, while similarity percentage represents the likelihood that the two proteins have evolved from a common ancestral protein. MegAlign Pro calculated the numerical values with the following parameters: distance metric = Uncorrected Pairwise; Gap treatment = Pairwise gap removal; Residues considered = 163–460.
| TMEM163 | ZnT1 | ZnT2 | ZnT3 | ZnT4 | ZnT5 | ZnT6 | ZnT7 | ZnT8 | ZnT9 | ZnT10 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| TMEM163 | 0 | 17.6 | 10.3 | 11.5 | 11.2 | 9.5 | 11.7 | 14.7 | 10.8 | 24.2 | 18.9 |
| ZnT1 | 36.5 | 0 | 30.5 | 27.1 | 25.8 | 25.6 | 16.1 | 26.5 | 27.3 | 14.5 | 38.9 |
| ZnT2 | 35.3 | 50.8 | 0 | 53.7 | 38.4 | 22.6 | 18.4 | 22.5 | 50.6 | 14.9 | 24.8 |
| ZnT3 | 28.6 | 45.4 | 67.5 | 0 | 35.1 | 20.5 | 20.2 | 22.3 | 44.5 | 13.4 | 22.7 |
| ZnT4 | 29.4 | 49.4 | 60.8 | 57.4 | 0 | 22.6 | 18.5 | 22.4 | 39.2 | 15.4 | 25.2 |
| ZnT5 | 32.1 | 48.8 | 45.0 | 42.5 | 44.2 | 0 | 24.3 | 43.6 | 21.6 | 16.0 | 25.9 |
| ZnT6 | 29.4 | 34.3 | 38.1 | 37.1 | 39.7 | 47.0 | 0 | 24.6 | 18.2 | 14.5 | 16.7 |
| ZnT7 | 36.8 | 48.8 | 46.4 | 42.6 | 47.8 | 63.0 | 44.5 | 0 | 21.8 | 15.4 | 27.8 |
| ZnT8 | 30.8 | 52.3 | 69.1 | 64.3 | 61.0 | 44.1 | 37.9 | 42.9 | 0 | 14.9 | 25.7 |
| ZnT9 | 46.1 | 36.6 | 36.1 | 31.4 | 34.0 | 34.4 | 30.1 | 35.2 | 33.4 | 0 | 17.3 |
| ZnT10 | 36.0 | 54.1 | 47.6 | 44.5 | 48.8 | 43.7 | 38.0 | 45.8 | 50.0 | 37.3 | 0 |