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. 2021 Feb 22;22(4):2179. doi: 10.3390/ijms22042179

Table 2.

Oligonucleotide primers used for RT-PCR analysis.

Gene Forward Reverse
ACTA2 5′-AATGGCTCTGGGCTCTGTAA-3′ 5′-TTTTGCTCTGTGCTTCGTCA-3′
FGFR1 5′-GGGCTGGAATACTGCTACAA-3′ 5′-GCCAAAGTCTGCTATCTTCATC-3′
FGFR2 5′-GGATAACAACACGCCTCTCTT-3′ 5′-GCCCAAAGCAACCTTCTC-3′
FGFR3 5′-TGGTGTCCTGTGCCTACC-3′ 5′-CCGTTGGTCGTCTTCTTGT-3′
FGFR4 5′-AACCGCATTGGAGGCATT-3′ 5′-TCTACCAGGCAGGTGTATGT-3′
GAPDH 5′-GAAGGTCGGAGTCAACGGATT-3′ 5′-TGACGGTGCCATGGAATTTG-3′
IL1 β 5′-GTGGCAATGAGGATGACTTG-3′ 5′-GTGGTGGTCGGAGATTCGTA-3′
IL6 5′-TGTGTGGGTCTGTTGTAGGG-3′ 5′-CCCGTGCAATATCTAGGAAAA-3′
IL8 5′-TGTGTGGGTCTGTTGTAGGG-3′ 5′-CCCGTGCAATATCTAGGAAAA-3′
INFγ 5′-GCAGGTCATTCAGATGTAGCGG-3′ 5′-CCACACTCTTTTGGATGCTCTGG-3′
MCP1 5′-CTCAGCCAGATGCAATCAA-3′ 5′-CACTTCTGCTTGGGGTCA-3′
NLRP3 5′-GGACTGAAGCACCTGTTGTGCA-3′ 5′-TCCTGAGTCTCCCAAGGCATTC-3′
RLBP1 5′-GCTGCTGGAGAATGAGGAAA-3′ 5′-TGGTGGATGAAGTGGATGG-3′
S100A4 5′-CCTGGATGTGATGGTGTCC-3′ 5′-TCGTTGTCCCTGTTGCTGT-3′
TNFα 5′-TGCTTGTTCCTCAGCCTCTT-3′ 5′-GCTTGTCACTCGGGGTTC-3′
VEGF-A 5′-AATCGAGACCCTGGTGGAC-3′ 5′-GGTGAGGTTTGATCCGCATA-3′
VEGFR1 5′-AGCAGTTCCACCACTTTAGA-3′ 5′-GAACTTTCCACAGAGCCCTT-3′
VEGFR2 5′-GGAAATGACACTGGAGCCTA-3′ 5′-TTTGAAATGGACCCGAGACA-3′
VEGFR3 5′-CAACGACCTACAAAGGCTCT-3′ 5′-GTAAAACACCTGGCCTCCTC-3′
VIM 5′-CGCCAGATGCGTGAAATG-3′ 5′-ACCAGAGGGAGTGAATCCAGA-3′

Abbreviations.ACTA2: actin α 2; FGFR: fibroblast growth factor receptor; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL: interleukin; INFγ: interferon γ; MCP1: monocyte chemoattractant protein 1; NLRP3: nucleotide-binding oligomerization domain (NOD), leucine-rich repeat (LRR)-containing proteins 3; RLBP1: retinaldehyde-binding protein 1; S100A4: S100 calcium-binding protein A4; TNFα: tumor necrosis factor α; VEGF-A: vascular endothelial growth factor-A; VEGFR: vascular endothelial growth factor receptor; VIM: vimentin.