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. 2021 Feb 23;10(2):412. doi: 10.3390/plants10020412

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phenotypes altered in selected Pseudomonas simiae PICF7 mutants. (A) Cop1 and Cop33 mutants are unable to grow on LB agar plates supplemented with 4.5 mM CuSO₄_·5H₂O (image taken 3 days after bacterial inoculation. (B) Mutants Bfm8 and Bfm9 are defective in producing biofilm. Upper image shows borosilicate tubes containing the bacterial cultures in LB medium. Lower image shows the result obtained after performing the staining procedure with crystal violet and the inner surface-adhered bacteria. (C) Mutants Phy17 and Phy18 are impaired in phytase activity when assayed in Phytase Specific Medium (PSM) (picture taken 3 days after bacterial inoculation). See the Materials and Methods section for full experimental details. PICF7, P. simiae PICF7; KT2440, Pseudomonas putida KT2440. These strains were used as positive controls. PIC128, Pseudomonas indica PIC128 was used as a negative control.