Table 1.

Disrupted genes identified in the selected Pseudomonas simiae PICF7 mutants.

Mutant Name	Length of the DNA Sequence Analyzed	Phenotype	Accession Number	Closest Strain	Query Cover (%)	E-Value	Identity (%)	Definition (GenBank)	Definition (GenName)
Cop1	294	Modified copper tolerance	WP_069672392.1	Pseudomonas simiae WCS417	61	1 × 10−31	100	chemotaxis protein CheV	cheV
Cop33	549		WP_176332083.1	Pseudomonas simiae WCS417	41	8 × 10−44	100	Heavy metal response regulator transcription factor	cusR
Bfm8	468	Impaired in biofilm formation	PHX42735.1	Pseudomonas simiae PCL1751	100	7 × 10−108	100	ATPase AAA	fleQ
Bfm9	212		WP_045791339.1	Pseudomonas simiae PCL1751	99	4 × 10−21	100	membrane protein	- 1

Identification of the disrupted genes by Tn5-Tc^R transposon insertion was carried out using a combination of arbitrary and nested-PCR strategy, followed by sequence comparison using the BLASTn and BLASTx algorithms available at the NCBI website. ¹ No definition provided.