Table 1.
Gene | GenBank Accession # | Source | Primer Sequence (5′-3′) | IVM Primer Efficiency (%) | IVC Primer Efficiency (%) | Product Size (bp) |
---|---|---|---|---|---|---|
GAPDH | NM_001034034.1 | [39] | 5′-ttcctggtacgacaatgaatttg-3′ 5′-ggagatggggcaggactc-3′ |
102.3 | 100.8 | 153 |
PPIA | NM_178320.2 | [10] | 5′-tcttgtccatggcaaatgctg-3′ 5′-tttcacctgccaaagtaccac-3′ |
98.8 | 99.0 | 111 |
Cx37 | NM_001083738.1 | [28] | 5′-gactcatctccctggtgctc-3′ 5′-gttctgctcactggacgaca-3′ |
97.0 | 100.3 | 221 |
Cx43 | NM_174068 | [40] | 5′-gtcttcgaggtggccttcttg-3′ 5′-agtccacctgatgtgggcag-3′ |
101.9 | NA | 104 |