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. 2021 Feb 23;12(2):321. doi: 10.3390/genes12020321

Table 1.

Primers used in qPCR.

Gene GenBank Accession # Source Primer Sequence (5′-3′) IVM Primer Efficiency (%) IVC Primer Efficiency (%) Product Size (bp)
GAPDH NM_001034034.1 [39] 5′-ttcctggtacgacaatgaatttg-3′
5′-ggagatggggcaggactc-3′
102.3 100.8 153
PPIA NM_178320.2 [10] 5′-tcttgtccatggcaaatgctg-3′
5′-tttcacctgccaaagtaccac-3′
98.8 99.0 111
Cx37 NM_001083738.1 [28] 5′-gactcatctccctggtgctc-3′
5′-gttctgctcactggacgaca-3′
97.0 100.3 221
Cx43 NM_174068 [40] 5′-gtcttcgaggtggccttcttg-3′
5′-agtccacctgatgtgggcag-3′
101.9 NA 104