Table 2.
Primers used in this study.
| Primer Names | Primer Sequences | Ta 1 (°C) | Fusarium Species/Toxin Producers Targeted | References |
|---|---|---|---|---|
| ßtub2a ßt-2 |
GGTAACCAAATCGGTGCTGCTTTC GATTGACCGAAAACGAAGTTG |
58 | Broad range 2 | [49,50,51] |
| Fum1-F Fum1-R |
GGATTGGCTGGATCTTCACAG GAAGATGGCATTGATTGCCTC |
57 | Fumonisins 3 | [52] |
| Tri5-TCTA-F Tri5-TCTA-R |
CTATTCCTTGAGATTACAT CCTTGTAGAATGACATAAGA |
58 | TCTA 4 | This paper |
| Tri5-TCTB-F Tri5-TCTB-R |
GATGGACACGATTGAGTG GCTCAAAGAACTTGCAGA |
58 | TCTB 5 | This paper |
| Fum1-656F Fum1-1158R |
CGGTTGTTCATCATCTCTGA GCTCCCGATGTAGAGCTTCTT |
60 | F. verticillioides | [53] |
| Fum1-656F Fum1-872R |
TGCTCGTCATCCCTGATAG GAAGATGGCATTGATTGCCTC |
60 | F. proliferatum | [52] |
| Fg16N-F Fg16N-R |
ACAGATGACAAGATTCAGGCACA TTCTTTGACATCTGTTCAACCCA |
60 | F. graminearum | [54] |
| FC01-F FC01-R |
ATGGTGAACTCGTCGTGGC CCCTTCTTACGCCAATCTCG |
60 | F. culmorum | [54] |
| FcroA-F FcroA-R |
CTCAGTGTCCACCGCGTTGCGTAG CTCAGTGTCCCAATCAAATAGTCC |
62 | F. cerealis | [54] |
| Fp1-F Fp1-R |
CGGGGTAGTTTCACATTTC(C/T)G GAGAATGTGATGA(C/G)GACAATA |
57 | F. pseudograminearum | [55] |
1 Annealing temperature; 2 Verified for the species acuminatum, asiaticum, austroamericanum, avenaceum, boothii, cerealis, compactum, cortaderiae, culmorum, equiseti, fujikuroi, graminearum, langsethiae, lateritium, lunulosporum, meridionale, mesoamericanum, oxysporum, poae, pseudograminerum, sacchari, sambucinum, sporotrichioides, subglutinans, tricinctum, temperatum, verticillioides, venenatum; 3 Verified for the species F. verticillioides, F. proliferatum, and F. fujikuroi; 4 Verified for the species acuminatum, equiseti, langsethiae, poae, sambucinum, sporotrichioides; 5 Verified for the species asiaticum, austroamericanum, boothii, cerealis, cortaderiae, culmorum, graminearum, lateritium, lunulosporum, meridionale, mesoamericanum, pseudograminerum.