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Detection of extracellular vesicle (EV)-related and overexpressed proteins after transfection. (A) Western blot analysis of overexpressed BAG6 (left) and B7-H6 (right) in HEK293 cells after 48 h of transfection. (B) BAG6 and B7-H6 were released into the supernatant (left), and BAG6 was detectable in EV lysates (right) 48 h after transfection. (C) Flotillin-1 was detected as an EV marker, and Calnexin was used as a negative control. WT = wildtype, SN = supernatant.
