Figure 4.

Cellular assays. (A) Bacterial growth assays. C. Rodentium, Enterohemorrhagic E. coli (EHEC), and S. enterica were cultured in LB media in the presence of YM155; bacterial growth was monitored as a function of time. (B) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. YM155 was added to RAW264.7 cells for 24 h and cell viability was assayed by monitoring MTT signal intensity. (C) Salmonella infection assays. RAW264.7 cells were seeded at 1 × 10⁵ cells/well in 24-well plates and YM155 was added 1 h before infection with 10⁶ CFUs of Salmonella for 30 min. * p < 0.05. Cells were incubated in medium containing 100 µg/mL gentamicin for 1 h, and then in 10 µg/mL gentamicin for an additional 23 h. Bacteria were released from RAW264.7 cells using 1% saponin, diluted in PBS, and plated for colony counts.