Table 1.

BLI-derived kinetic parameters describing the interactions of suramin with hRKIP and its mutants.

Protein	KD (×10−5 M)	kon (M−1s−1)	koff (×10−4 s−1)	kobs (×10−4 s−1)	Full R2 (×10−1)
RKIP	2.38	8.69	2.07	36.8	9.68
P74L	3.71	7.39	2.75	3.67	9.92
Y81A	2.97	5.31	1.58	2.91	9.94
S109A	2.60	6.93	1.80	29.5	9.90
P112L	2.04	8.14	1.66	3.70	9.57
K113A	8.72	3.38	2.95	3.80	8.15
W173A	7.73	3.12	2.41	5.53	9.76
Y181A	71.1	0.22	1.56	1.77	9.63