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. 2021 Feb 23;10(2):480. doi: 10.3390/cells10020480

Figure 4.

Figure 4

Compound 68 reduced LPS-induced TRAF6 and LRRK2 interaction. Human lymphoblasts were incubated in serum-free medium for 12 h and then exposed to LPS (1 µg/mL) with or without pre-treating 68 (10 µM) for 2 h, then kept treatment for various time periods as indicated. Cell lysates were harvested at the indicated time points after LPS exposure, then were subjected to co-IP assays using an anti-LRRK2 antibody, followed by anti-TRAF6 and anti-LRRK2 immunoblotting. LRRK2 antibody pulled down TRAF6. LPS increased LRRK2 interaction with TRAF6, while 68 attenuated this interaction. (A) Representative blots from the three separate experiments. (B) The quantification of LRRK2 interaction with TRAF6 at 30 min post LPS exposure (peak time). * p < 0.05 by ANOVA, vs. untreated cells at 0 min # p < 0.05 by ANOVA, vs. cells at 30 min post LPS exposure.