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. 2021 Feb 23;10(2):480. doi: 10.3390/cells10020480

Figure 5.

Figure 5

Compound 68 reduced LPS-induced TRAF6-linked MAPK signaling pathways. Human lymphoblasts were incubated in serum-free medium for 12 h and then exposed to LPS (1 µg/mL) with or without pre-treating 68 (10 µM) for 2 h, then kept treatment for various time periods as indicated. Cell lysates were harvested at the indicated time points after LPS exposure, then were subjected to immunoblot analysis using indicated antibodies. (A) Representative blots from the three separate experiments. (B–D). The quantification of phosphorylated pJNK 1/2 (B), p38 (C), and pERK1/2 levels at peak time. * p < 0.05 by ANOVA, vs. untreated cells at 0 min. # p < 0.05 by ANOVA, vs. cells at 4 h post LPS exposure.