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. 2021 Feb 22;26(4):1156. doi: 10.3390/molecules26041156

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Amount of neutral lipids in primary human hepatocytes (PHHs) after treatment with free fatty acids (FFAs). PHHs were treated with 1 mM FFAs for 24 h and lipid levels were quantified using the Oil Red O assay normalized to the protein amount measured by sulforhodamine B (SRB) assay. (A) Evaluation of steatosis in FFA-treated PHHs in comparison to control. Data are shown as the mean + SD, n = 5, paired t-test, p < 0.01 (**). (B) Microscopic evaluation of the lipid accumulation in representative PHH cultures (magnification 200×), the scale bar is 10 µm.