Table 3.
qPCR thermocycling conditions.
| Step | Temperature ( °C) | Time (min:s) | Cycles |
|---|---|---|---|
| UNG 1 incubation | 50 | 2:00 | 1 |
| Polymerase activation | 95 | 10:00 | 1 |
| Denaturation | 95 | 0:15 | 40 |
| Annealing/extension | 60 | 1:00 |
1 Uracil-N-glycosylase.
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