Table 3.
Chemical constituents of the hexane extracts of cell suspension culture (CSC) and plant of Eysenhardtia platycarpa identified by GC‒MS.
| Compound Name | Chemical Formula | RT (min) | Abundance (%) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Fatty Hexane Extract | Defatted Hexane Extract | |||||||||
| Sapwood | Heartwood | Leaf | CSC | Sapwood | Heartwood | Leaf | CSC | |||
| Alkanes | ||||||||||
| Heptacosane | C27H56 | 38.38 | - | - | 2.46 ± 0.02 | - | - | - | - | - |
| Nonacosane | C29H60 | 42.98 | - | - | 6.36 ± 0.01 | - | - | - | - | - |
| Octacosane | C28H58 | 47.26 | - | - | 1.31 ± 0.02 | - | - | - | - | - |
| Aromatic compounds | ||||||||||
| Isophthalic acid | C8H6O4 | 13.9 | 1.19 ± 0.09 | - | - | - | - | - | - | - |
| Terephthalic acid | C8H6O4 | 14.72 | 1.30 ± 0.23 | - | - | - | - | - | - | - |
| Dibutyl phthalate | C16H22O4 | 19.14 | - | 5.49 ± 0.60 | 0.69 ± 0.06 | 3.91 ± 0.03 | 2.51 ± 0.05 | 3.91 ± 0.01 | 1.05 ± 0.05 | 2.48 ± 0.13 |
| 1,2-Dihydroxyanthraquinone | C14H8O4 | 31.87 | - | - | - | - | - | - | 0.28 ± 0.04 | - |
| Bis(2-ethylhexyl) phthalate | C24H38O4 | 34.69 | - | - | - | - | - | - | - | 0.79 ± 0.02 |
| 4,5-Dihydroxyanthraquinone-2-carboxylic acid | C15H8O6 | 41.37 | - | - | 0.47 ± 0.05 | - | - | - | - | - |
| Derivatives from saturated alkanoic acids | ||||||||||
| 2-Hydroxyheptanoic acid | C7H14O3 | 6.41 | 1.23 ± 0.50 | 1.40 ± 0.05 | - | - | - | - | - | - |
| 3-Phenylpropanoic acid | C9H10O2 | 7.12 | - | - | 4.38 ± 0.09 | - | - | - | 6.28 ± 0.33 | - |
| 3-Hydroxyoctanoic acid | C8H16O3 | 7.98 | 0.78 ± 0.06 | 0.80 ± 0.02 | - | - | - | - | - | - |
| 3-(4-Methoxyphenyl) propionic acid | C10H12O3 | 11.49 | - | - | 2.34 ± 0.11 | - | - | - | 2.48 ± 0.13 | - |
| 2,3-Dihydroxypropyl palmitate | C19H38O4 | 36.21 | - | - | - | 0.60 ± 0.04 | - | - | - | 0.53 ± 0.00 |
| Derivatives from unsaturated alkanoic acids | ||||||||||
| 3-Phenylprop-2-enoic acid | C9H8O2 | 9.2 | - | - | 0.55 ± 0.02 | - | - | - | 0.76 ± 0.06 | - |
| Saturated alkanoic acids | ||||||||||
| Tetradecanoic acid | C14H28O2 | 16.07 | - | - | 1.06 ± 0.02 | 0.39 ± 0.00 | 0.20 ± 0.01 | - | 1.56 ± 0.07 | 0.42 ± 0.00 |
| Pentadecanoic acid | C15H30O2 | 18.74 | - | - | - | 0.42 ± 0.01 | - | - | - | 0.33 ± 0.03 |
| Hexadecanoic acid | C16H32O2 | 21.49 | 14.32 ± 0.45 | 10.06 ± 0.13 | 11.48 ± 0.12 | 62.58 ± 0.36 | 6.25 ± 0.08 | 3.37 ± 0.03 | 19.02 ± 0.02 | 63.01 ± 0.07 |
| Heptadecanoic acid | C17H34O2 | 24.22 | - | - | - | 0.85 ± 0.01 | 0.32 ± 0.00 | 0.17 ± 0.02 | - | 0.80 ± 0.00 |
| Octadecanoic acid | C18H36O2 | 26.93 | 1.90 ± 0.09 | 1.49 ± 0.07 | 2.93 ± 0.08 | 6.09 ± 0.00 | 3.11 ± 0.05 | 0.70 ± 0.01 | 5.14 ± 0.10 | 6.91 ± 0.06 |
| Eicosanoic acid | C20H40O2 | 32.17 | - | - | 0.55 ± 0.00 | 1.14 ± 0.00 | - | 0.14 ± 0.01 | 0.92 ± 0.03 | 1.00 ± 0.01 |
| Saturated alkanoic acids | ||||||||||
| Docosanoic acid | C22H44O2 | 37.08 | - | - | 1.19 ± 0.01 | 0.84 ± 0.00 | - | - | 1.51 ± 0.05 | 0.61 ± 0.01 |
| Tetracosanoic acid | C24H48O2 | 41.71 | - | - | 5.09 ± 0.03 | 2.81 ± 0.01 | - | - | 3.95 ± 0.05 | 2.15 ± 0.05 |
| Saturated diacids | ||||||||||
| Hexanedioic acid | C6H10O4 | 8.49 | 0.91 ± 0.01 | 1.79 ± 0.53 | - | - | - | 0.19 ± 0.04 | - | - |
| Heptanedioic acid | C7H12O4 | 10.33 | 1.54 ± 0.02 | - | - | - | - | - | - | - |
| Octanedioic acid | C8H14O4 | 12.44 | 5.41 ± 0.36 | 2.90 ± 0.34 | - | 0.96 ± 0.09 | 0.18 ± 0.00 | 0.21 ± 0.02 | - | 0.82 ± 0.05 |
| Nonanedioic acid | C9H16O4 | 14.87 | 27.93 ± 1.48 | 16.22 ± 1.27 | 1.02 ± 0.02 | 4.18 ± 0.03 | 0.94 ± 0.04 | 1.03 ± 0.01 | 0.92 ± 0.05 | 3.97 ± 0.10 |
| Steroids | ||||||||||
| Campesterol | C28H48O | 50.45 | - | - | - | - | 8.76 ± 0.07 | 9.15 ± 0.20 | - | - |
| Stigmasterol | C29H48O | 51.14 | - | 1.19 ± 0.14 | - | 0.55 ± 0.08 | 23.56 ± 0.19 | 23.50 ± 0.11 | 1.55 ± 0.02 | 2.58 ± 0.08 |
| β-Sitosterol | C29H50O | 52.32 | - | - | - | - | 43.76 ± 0.03 | 43.43 ± 0.14 | - | 1.87 ± 0.01 |
| Stigmastanol | C29H52 | 52.43 | - | - | - | - | 1.30 ± 0.12 | 1.04 ± 0.08 | - | 1.42 ± 0.02 |
| Stigmasta-3,5-dien-7-one | C29H46O | 53.27 | 17.48 ± 0.35 | 19.00 ± 0.29 | - | - | - | - | - | - |
| Sitostenone | C29H48O | 54.05 | - | - | - | - | - | 0.57 ± 0.04 | - | - |
| Triterpenoids | ||||||||||
| β-Amyrin | C30H50O | 52.16 | - | - | 7.13 ± 0.46 | - | - | - | 16.30 ± 0.49 | - |
| Unsaturated alkanoic acids | ||||||||||
| (Z,Z)-9,12-Octadecadienoic acid | C18H32O2 | 25.99 | - | - | 0.67 ± 0.01 | - | - | - | 2.46 ± 0.06 | - |
| (Z,Z,Z)-9,12,15-Octadecatrienoic acid | C18H30O2 | 26.17 | - | - | 1.49 ± 0.01 | - | - | - | 6.88 ± 0.16 | - |
| (Z)-9-Octadecenoic acid | C18H34O2 | 26.18 | - | - | - | - | 0.25 ± 0.01 | 0.25 ± 0.01 | - | - |
| Others | ||||||||||
| 6,10,14-Trimethylpentadecan-2-one | C18H36O | 15.91 | - | - | 0.53 ± 0.03 | - | - | - | 0.66 ± 0.03 | - |
| 3,7,11,15-Tetramethyl-2-hexadecen-1-ol | C20H40O | 25.11 | - | - | - | - | - | - | 2.89 ± 0.15 | - |
| Octacosanol | C28H58O | 48.27 | - | - | 22.68 ± 0.01 | - | - | - | 3.85 ± 0.13 | - |
Values represent the mean ± standard deviation of two replicates.