Table 4.
Chemical constituents of the Dichloromethane and methanolic extracts of cell suspension culture (CSC) and plant of Eysenhardtia platycarpa identified by GC‒MS.
| Compound Name | Chemical Formula | RT(min) | Abundance (%) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dichloromethane Extract | Methanolic Extract | |||||||||
| Sapwood | Heartwood | Leaf | CSC | Sapwood | Heartwood | Leaf | CSC | |||
| Aromatic compounds | ||||||||||
| Vanillin | C8H8O3 | 9.09 | 0.53 ± 0.32 | - | - | - | - | - | - | - |
| Terephthalic acid | C8H6O4 | 14.72 | 0.39 ± 0.12 | - | - | - | - | - | - | - |
| Dibutyl phthalate | C16H22O4 | 19.14 | 3.05 ± 0.82 | 2.97 ± 0.16 | - | 0.56 ± 0.01 | - | - | - | - |
| 1,2-Dihydroxyanthraquinone | C14H8O4 | 31.87 | - | 1.58 ± 0.01 | - | 0.43 ± 0.03 | - | - | - | - |
| Derivatives from saturated alkanoic acids | ||||||||||
| 3-Phenylpropanoic acid | C9H10O2 | 7.12 | - | - | 1.02 ± 0.00 | - | - | - | - | - |
| 3-(4-Methoxyphenyl) propionic acid | C10H12O3 | 11.49 | - | - | 0.65 ± 0.02 | - | - | - | - | - |
| 2,3-Dihydroxypropyl palmitate | C19H38O4 | 36.21 | 1.19 ± 0.00 | 0.72 ± 0.01 | - | 0.96 ± 0.01 | - | - | - | - |
| Mono and disaccharides | ||||||||||
| Ketohexoses | C6H12O6 | * | - | - | - | - | 10.12 ± 0.10 | 3.14 ± 0.08 | 3.61 ± 0.02 | 27.77 ± 0.03 |
| Aldohexoses | C6H12O6 | * | - | - | - | - | 6.30 ± 0.32 | 3.49 ± 0.24 | 2.88 ± 0.07 | 2.79 ± 0.00 |
| Furanoses | C6H12O6 | * | - | - | - | - | 4.67 ± 0.08 | - | 0.96 ± 0.02 | - |
| Disaccharides (glucose with fructose) | C12H22O11 | * | - | - | - | - | 28.24 ± 0.44 | 3.55 ± 0.12 | 1.82 ± 0.00 | 40.91 ± 0.09 |
| Polyols | ||||||||||
| L-threitol | C4H10O4 | 8.77 | - | - | - | - | - | - | - | 0.42 ± 0.00 |
| Xylitol | C5H12O5 | 13.52 | - | - | - | - | 0.40 ± 0.00 | 4.23 ± 0.15 | - | 2.12 ± 0.00 |
| D-pinitol | C7H14O6 | 16.38 | - | - | - | - | 37.00 ± 0.22 | 15.56 ± 0.47 | 74.28 ± 0.01 | - |
| Ribitol | C5H12O5 | 18.11 | - | - | - | - | - | - | - | 9.22 ± 0.01 |
| Sorbitol | C6H14O6 | 19.21 | - | - | - | - | - | 2.07 ± 0.08 | - | 1.84 ± 0.00 |
| Inositol | C6H12O6 | * | - | - | - | - | 7.07 ± 0.05 | 7.00 ± 0.22 | 6.46 ± 0.02 | 5.22 ± 0.01 |
| Saturated diacids | ||||||||||
| Hexanedioic acid | C6H10O4 | 8.49 | - | 0.48 ± 0.02 | - | - | - | - | - | - |
| Octanedioic acid | C8H14O4 | 12.44 | - | 0.74 ± 0.10 | - | - | - | - | - | - |
| Nonanedioic acid | C9H16O4 | 14.87 | 1.94 ± 0.09 | 4.53 ± 0.73 | 0.51 ± 0.01 | 0.63 ± 0.01 | 0.44 ± 0.09 | 1.17 ± 0.49 | - | - |
| Saturated alkanoic acids | ||||||||||
| Tetradecanoic acid | C14H28O2 | 16.07 | - | - | 0.36 ± 0.01 | - | - | - | - | - |
| Hexadecanoic acid | C16H32O2 | 21.49 | 27.42 ± 0.26 | 22.40 ± 0.26 | 5.20 ± 0.00 | 35.02 ± 0.44 | - | - | 2.06 ± 0.00 | 2.05 ± 0.00 |
| Heptadecanoic acid | C17H34O2 | 24.22 | 0.77 ± 0.13 | 0.87 ± 0.02 | - | 0.36 ± 0.00 | - | - | - | - |
| Octadecanoic acid | C18H36O2 | 26.93 | 3.37 ± 0.21 | 3.21 ± 0.04 | 1.44 ± 0.00 | 2.75 ± 0.22 | - | - | 1.88 ± 0.01 | 1.16 ± 0.03 |
| Eicosanoic acid | C20H40O2 | 32.17 | 0.58 ± 0.04 | - | - | 0.42 ± 0.00 | - | - | - | - |
| Docosanoic acid | C22H44O2 | 37.08 | 0.54 ± 0.01 | - | - | 0.29 ± 0.01 | - | - | - | - |
| Tetracosanoic acid | C24H48O2 | 41.71 | 1.25 ± 0.10 | 0.90 ± 0.00 | 0.96 ± 0.03 | 1.27 ± 0.01 | - | - | - | - |
| Sesquiterpenoids | ||||||||||
| β-Selinene | C15H24 | 8.31 | - | - | 0.46 ± 0.01 | - | - | - | - | - |
| γ-Muurolene | C15H24 | 8.74 | - | - | 0.32 ± 0.01 | - | - | - | - | - |
| β-Cadinene | C15H24 | 11.2 | - | - | 0.77 ± 0.03 | - | - | - | - | - |
| 11-Hydroxy-4βH,5α-eremophil-1(10)-ene | C15H26O | 13.42 | - | - | 0.60 ± 0.02 | - | - | - | - | - |
| Trans, trans-farnesol | C15H26O | 15.07 | - | - | 0.49 ± 0.01 | - | - | - | - | - |
| Steroids | ||||||||||
| Campesterol | C28H48O | 50.45 | 1.61 ± 0.11 | 1.76 ± 0.11 | - | 0.82 ± 0.11 | - | - | - | - |
| Stigmasterol | C29H48O | 51.14 | 4.06 ± 0.41 | 3.96 ± 0.16 | - | 6.72 ± 0.05 | - | - | - | - |
| β-Sitosterol | C29H50O | 52.32 | 8.19 ± 0.63 | 7.50 ± 0.55 | - | 6.00 ± 0.07 | - | - | - | - |
| Unsaturated alkanoic acids | ||||||||||
| (Z,Z)-9,12-Octadecadienoic acid | C18H32O2 | 25.99 | 3.99 ± 0.06 | 5.20 ± 0.05 | 0.72 ± 0.03 | 13.63 ± 0.16 | - | - | - | - |
| (Z,Z,Z)-9,12,15-Octadecatrienoic acid, | C18H30O2 | 26.17 | - | - | 2.29 ± 0.00 | 3.79 ± 0.06 | - | - | - | - |
| (Z)-9-Octadecenoic acid | C18H34O2 | 26.18 | 1.62 ± 0.04 | 2.49 ± 0.05 | - | 0.82 ± 0.01 | - | - | - | - |
| Others | ||||||||||
| Malic acid | C4H6O5 | 8.3 | - | - | - | - | 1.95 ± 0.01 | - | - | - |
| L-Threonic acid | C4H8O5 | 9.79 | - | - | - | - | - | - | 0.42 ± 0.00 | - |
| Galactaric acid | C6H10O8 | 20.16 | - | - | - | - | - | 1.68 ± 0.06 | - | - |
| 3,7,11,15-Tetramethyl-2-hexadecen-1-ol | C20H40O | 25.11 | - | - | 1.06 ± 0.00 | - | - | - | - | - |
Values represent the mean ± standard deviation of two replicates. * Several stereoisomers were found at different retention times.