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Inducible α-syn expression in differentiated SH-SY5Y cells: experimental design. Schematic representation of the timeline of SH-SY5Y differentiation and sEV treatment. Bright-field and fluorescent images (20× and 40× magnification, respectively) show the progressive development of a neuron-like phenotypes in these cell lines and the increase in α-syn expression after tetracycline treatment, as also confirmed by Western blot images.
