Figure 3.

Effect of patient-derived sEVs on α-syn expression in SH-SY5Y cells. (A) Total α-syn levels in SH-SY5Y cells after treatment with sEVs derived from non-mutated (NM-PD) and GBA-mutated (GBA-PD) PD patients, carrying mild (N370S) or severe (L444P) mutations. (B) Expression levels and representative images of fluorescent signal of phospho-syn (Ser129) in SH-SY5Y cells after treatment with patient-derived sEVs. The expression of α-syn (total and phosphorylated form) is normalized with the housekeeping protein (β-actin) and reported as a percentage compared with α-syn expression in SH-SY5Y cells treated with healthy control (HC)-derived sEVs. All data were expressed as mean ± SEM of three independent experiments. Representative images of proteins blots are reported.