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. 2021 Feb 23;9(2):459. doi: 10.3390/microorganisms9020459

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The gene mcwc-1a is essential to maintain the virulence potential. Virulence assays in immunosuppressed mice injected with 1 × 10⁶ spores of the mutants in the three wc-1 genes of M. lusitanicus: mcwc-1aΔ (blue), mcwc-1bΔ (purple), and mcwc-1cΔ (orange). In addition, two control strains were also injected: the virulent wild-type strain R7B (red) and the avirulent wild-type strain NRRL3631 (green). The survival rate of the mutant was compared to the virulent control strain and statistically analyzed by a Mantel–Cox test (*, p-value = 0.006).