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. 2021 Feb 23;10(2):334. doi: 10.3390/antiox10020334

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Validation of expression and/or secretion of the most differentially upregulated SASP and interferon/STAT signaling related genes that were identified in the transcriptomics analysis of senescent preadipocytes. (A) Gene expression analysis of the most significantly upregulated putative SASP molecules in senescent 3T3-L1 preadipocytes (Day 7) compared to untreated control by real-time quantitative polymerase chain reaction (qPCR). Results (fold change vs. control) are presented as means ± SEM from three independent experiments. * p ≤ 0.05, *** p ≤ 0.001, **** p ≤ 0.0001 (Student’s t-test). (B) Graph represents data for ImageJ quantification of the intensity of the signal of the indicated secreted molecule in conditioned media of senescent preadipocytes compared to untreated cells. Data are means ± SEM from 3 independent experiments. * p ≤ 0.05 (Student’s t-test).